Serum
Client-Provided Antigen
Time: ~13 weeks
13 weeks
Immunization Protocol
- 1 Sheep/goat
- 1 Pre-immune serum collection
- 4 Immunizations per sheep/goat
- 4 Production serum collections per sheep/goat
Deliverables
- Pre-immune serum, ~25 ml each sheep/goat
- 4 Production serum collections, ~150-250 ml each sheep/goat
Peptide
ProSci-Provided Antigen
Time: ~16-17 weeks
3-4 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis of up to 15 aa
- Conjugation to carrier protein
13 weeks
Immunization Protocol
- 1 Sheep/goat
- 1 Pre-immune serum collection
- 4 Immunizations per sheep/goat
- 4 Production serum collections per sheep/goat
Deliverables
- Pre-immune serum, ~25 ml each sheep/goat
- 4 Production serum collections, ~150-250 ml each sheep/goat
- 5 mg peptide
- ELISA report
Purified
ProSci-Provided Antigen + Serum
Purification
Time: ~17-19 weeks
3-4 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis of up to 15 aa
- Conjugation to carrier protein
13 weeks
Immunization Protocol
- 1 Sheep/goat
- 1 Pre-immune serum collection
- 4 Immunizations per sheep/goat
- 4 Production serum collections per sheep/goat
1-2 weeks
Purification
- Immuno-affinity purification of 25 ml
serum against peptide
Deliverables
- Pre-immune serum, ~25 ml each sheep/goat
- 4 Production serum collections, ~150-250 ml each sheep/goat
- 5 mg peptide
- ELISA report
- Purified antibody
- Immuno-affinity column
- Purification report
