Serum
Client-Provided Antigen
Time: ~8 weeks
8 weeks
Immunization Protocol
- 5 Mice
- 1 Pre-immune serum collection
- 4 Immunizations per mouse
- 1 Test serum collection
- 1 Production serum collection
Deliverables
- Pre-immune serum, ~0.05 ml each mouse
- Test serum, ~0.05 ml each mouse
- Production serum collection, ~0.5ml each mouse
Peptide
ProSci-Provided Antigen
Time: ~11-12 weeks
3-4 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis of up to 15 aa
- Conjugation to carrier protein
8 weeks
Immunization Protocol
- 5 Mice
- 1 Pre-immune serum collection
- 4 Immunizations per mouse
- 1 Test serum collection
- 1 Production serum collection
Deliverables
- Pre-immune serum, ~0.05 ml each mouse
- Test serum, ~0.05 ml each mouse
- Production serum collection, ~0.5ml each mouse
- 5 mg peptide
- ELISA report
Purified
ProSci-Provided Antigen + Serum
Purification
Time: ~12-14 weeks
3-4 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis of up to 15 aa
- Conjugation to carrier protein
8 weeks
Immunization Protocol
- 5 Mice
- 1 Pre-immune serum collection
- 4 Immunizations per mouse
- 1 Test serum collection
- 1 Production serum collection
1-2 weeks
Purification
- Immuno-affinity purification of serum against peptide
Deliverables
- Pre-immune serum, ~0.05 ml each mouse
- Test serum, ~0.05 ml each mouse
- 5 mg peptide
- ELISA report
- Purified antibody
- Immuno-affinity column
- Purification report