IgY
Client-Provided Antigen
Time: ~9-10 weeks
~11-12 weeks
Immunization Protocol
- 2 Chickens
- Pre-immune IgY purification
- 4 Immunizations per chicken
- 3 Test serum collections per chicken
- IgY purification of eggs collected weeks 5-8
Deliverables
- 5 mg pre-immune IgY
- 3 test serum samples, ~1 ml each chicken
- ~1000 mg immune IgY
Peptide
ProSci Provided Antigen
Time: ~11-13 weeks
3-4 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis of up to 15 aa
- Conjugation to carrier protein
11-12 weeks
Immunization Protocol
- 2 Chickens
- 4 Immunizations per chicken
- 3 test serum collections per chicken
- IgY purification of eggs collected weeks 5-8
Deliverables
- 3 test sera samples, ~1 ml each chicken
- ~1000 mg immune IgY
- 5 mg peptide
- ELISA report
Purified
ProSci-Provided Antigen + Yolk
Purification
Time: ~12-15 weeks
3-4 weeks
Antigen Design & Synthesis
- Antigen design
- Peptide synthesis of up to 15 aa
- Conjugation to carrier protein
11-12 weeks
Immunization Protocol
- 2 chickens
- 4 immunizations per chicken
- 3 test serum collections per chicken
- IgY purification of eggs collected weeks 5-8
1-2 weeks
Immuno-Affinity Purification
- Immuno-affinity purification of 25 mg IgY against peptide
Deliverables
- 3 Test sera samples, ~1 ml each chicken
- ~1000 mg immune IgY
- 5 mg peptide
- ELISA report
- Purified antibody
- Immuno-affinity column
- Purification report
